Virus:Article Title: CDK1-PP2A-B55 interplay ensures cell cycle oscillation via Apc1-loop 300 .
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Apc1 (pS314/pS318) Lab stock #25312 via BioGenes Rabbit polyclonal anti-Apc1 (pS358) This paper #27644 via BioGenes Rabbit polyclonal anti-Apc1 (pT378/pS380) This paper #25316 via BioGenes Rabbit polyclonal anti-Apc1 Lab stock #4853 via BioGenes Rabbit polyclonal anti-Apc2 Lab stock #7576 and #7577 via BioGenes Mouse monoclonal anti-Cdc27/Apc3 BD Biosciences 610455; RRID:AB_397828 Mouse monoclonal anti-Apc3 (AF3.1) Gift from J. Gannon N/A Rabbit polyclonal anti-Apc5 Lab stock #3445 via BioGenes Rabbit polyclonal anti-Apc6 Lab stock #3447 via BioGenes Rabbit polyclonal anti-Gwl Lab stock #7387 via BioGenes Rabbit polyclonal anti-XErp1 Lab stock #8344 via BioGenes Mouse monoclonal anti-FLAG (M2) Sigma-Aldrich F1804; RRID:AB_262044 Mouse monoclonal anti-Cyclin B (X121) Gift from J. Gannon N/A Mouse monoclonal anti-Cdc20 (BA8.1) Gift from J. Gannon N/A Mouse monoclonal anti-6xHis Clontech Cat#631212; RRID:AB_2721905 Mouse monoclonal anti-phosphorylated Plx1 (AZ44) Gift from J. Gannon N/A Goat anti-mouse immunoglobulins, HRPconjugated Dako Cat#P0447; RRID:AB_2617137 Swine anti-rabbit immunoglobulins, HRPconjugated Dako Cat#P0217; RRID:AB_2728719 IRDye 680RD goat anti-mouse immunoglobulins LI-COR Biosciences Cat#926–68070; RRID:AB_2651128 IRDye 800CW goat anti-rabbit immunoglobulins LI-COR Biosciences Cat#926–32211; RRID:AB_2651127 Bacterial and virus strains Escherichia coli DH5a Lab stock N/A Escherichia coli BL21-CodonPlus (DE3)-RIL Agilent Cat#230245 Multibac baculovirus/insect cell system Zhang Z. et al.40 and Fujimitsu K. et al.15 N/A Chemicals, peptides, and recombinant proteins ATP Sigma-Aldrich Cat#10519979001 ATPgS Calbiochem Cat#119120 [g-P32]ATP, 6000 Ci/mmol, 10 mCi/mL Hartmann Analytic SRP-501 [S35]Met-label, 1000 Ci/mmol, 10 mCi/mL Hartmann Analytic SCIS-103 Cycloheximide Sigma-Aldrich Cat#C7698 Okadaic acid Sigma-Aldrich Cat#O4511 MG132 proteasome inhibitor Calbiochem Cat#474790 Desthiobiotin Sigma-Aldrich Cat#D1411 Lysozyme Sigma-Aldrich Cat#L6876 Benzonase MILLIPORE Cat#70746 Methylated ubiquitin Sigma-Aldrich Cat#662065 3FLAG-Ub-Cyclin B-N70-KA (Ub-K0-Cyclin B) Fujimitsu K. and Yamano H.41 N/A GST-Cyclin BD167 Gift from J. Gannon pGEX Hs cyclin BD167 CDK2-Cyclin A complex Gift from J. Gannon N/A GST-PreScission protease Lab stock N/A 10His-ENSA Lab stock Plasmid stock #1523 (Continued on next page) Cell Reports 43, 114155, May 28, 2024 11
Recombinant:Article Title: CDK1-PP2A-B55 interplay ensures cell cycle oscillation via Apc1-loop 300 .
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Apc1 (pS314/pS318) Lab stock #25312 via BioGenes Rabbit polyclonal anti-Apc1 (pS358) This paper #27644 via BioGenes Rabbit polyclonal anti-Apc1 (pT378/pS380) This paper #25316 via BioGenes Rabbit polyclonal anti-Apc1 Lab stock #4853 via BioGenes Rabbit polyclonal anti-Apc2 Lab stock #7576 and #7577 via BioGenes Mouse monoclonal anti-Cdc27/Apc3 BD Biosciences 610455; RRID:AB_397828 Mouse monoclonal anti-Apc3 (AF3.1) Gift from J. Gannon N/A Rabbit polyclonal anti-Apc5 Lab stock #3445 via BioGenes Rabbit polyclonal anti-Apc6 Lab stock #3447 via BioGenes Rabbit polyclonal anti-Gwl Lab stock #7387 via BioGenes Rabbit polyclonal anti-XErp1 Lab stock #8344 via BioGenes Mouse monoclonal anti-FLAG (M2) Sigma-Aldrich F1804; RRID:AB_262044 Mouse monoclonal anti-Cyclin B (X121) Gift from J. Gannon N/A Mouse monoclonal anti-Cdc20 (BA8.1) Gift from J. Gannon N/A Mouse monoclonal anti-6xHis Clontech Cat#631212; RRID:AB_2721905 Mouse monoclonal anti-phosphorylated Plx1 (AZ44) Gift from J. Gannon N/A Goat anti-mouse immunoglobulins, HRPconjugated Dako Cat#P0447; RRID:AB_2617137 Swine anti-rabbit immunoglobulins, HRPconjugated Dako Cat#P0217; RRID:AB_2728719 IRDye 680RD goat anti-mouse immunoglobulins LI-COR Biosciences Cat#926–68070; RRID:AB_2651128 IRDye 800CW goat anti-rabbit immunoglobulins LI-COR Biosciences Cat#926–32211; RRID:AB_2651127 Bacterial and virus strains Escherichia coli DH5a Lab stock N/A Escherichia coli BL21-CodonPlus (DE3)-RIL Agilent Cat#230245 Multibac baculovirus/insect cell system Zhang Z. et al.40 and Fujimitsu K. et al.15 N/A Chemicals, peptides, and recombinant proteins ATP Sigma-Aldrich Cat#10519979001 ATPgS Calbiochem Cat#119120 [g-P32]ATP, 6000 Ci/mmol, 10 mCi/mL Hartmann Analytic SRP-501 [S35]Met-label, 1000 Ci/mmol, 10 mCi/mL Hartmann Analytic SCIS-103 Cycloheximide Sigma-Aldrich Cat#C7698 Okadaic acid Sigma-Aldrich Cat#O4511 MG132 proteasome inhibitor Calbiochem Cat#474790 Desthiobiotin Sigma-Aldrich Cat#D1411 Lysozyme Sigma-Aldrich Cat#L6876 Benzonase MILLIPORE Cat#70746 Methylated ubiquitin Sigma-Aldrich Cat#662065 3FLAG-Ub-Cyclin B-N70-KA (Ub-K0-Cyclin B) Fujimitsu K. and Yamano H.41 N/A GST-Cyclin BD167 Gift from J. Gannon pGEX Hs cyclin BD167 CDK2-Cyclin A complex Gift from J. Gannon N/A GST-PreScission protease Lab stock N/A 10His-ENSA Lab stock Plasmid stock #1523 (Continued on next page) Cell Reports 43, 114155, May 28, 2024 11
Methylation:Article Title: CDK1-PP2A-B55 interplay ensures cell cycle oscillation via Apc1-loop 300 .
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Apc1 (pS314/pS318) Lab stock #25312 via BioGenes Rabbit polyclonal anti-Apc1 (pS358) This paper #27644 via BioGenes Rabbit polyclonal anti-Apc1 (pT378/pS380) This paper #25316 via BioGenes Rabbit polyclonal anti-Apc1 Lab stock #4853 via BioGenes Rabbit polyclonal anti-Apc2 Lab stock #7576 and #7577 via BioGenes Mouse monoclonal anti-Cdc27/Apc3 BD Biosciences 610455; RRID:AB_397828 Mouse monoclonal anti-Apc3 (AF3.1) Gift from J. Gannon N/A Rabbit polyclonal anti-Apc5 Lab stock #3445 via BioGenes Rabbit polyclonal anti-Apc6 Lab stock #3447 via BioGenes Rabbit polyclonal anti-Gwl Lab stock #7387 via BioGenes Rabbit polyclonal anti-XErp1 Lab stock #8344 via BioGenes Mouse monoclonal anti-FLAG (M2) Sigma-Aldrich F1804; RRID:AB_262044 Mouse monoclonal anti-Cyclin B (X121) Gift from J. Gannon N/A Mouse monoclonal anti-Cdc20 (BA8.1) Gift from J. Gannon N/A Mouse monoclonal anti-6xHis Clontech Cat#631212; RRID:AB_2721905 Mouse monoclonal anti-phosphorylated Plx1 (AZ44) Gift from J. Gannon N/A Goat anti-mouse immunoglobulins, HRPconjugated Dako Cat#P0447; RRID:AB_2617137 Swine anti-rabbit immunoglobulins, HRPconjugated Dako Cat#P0217; RRID:AB_2728719 IRDye 680RD goat anti-mouse immunoglobulins LI-COR Biosciences Cat#926–68070; RRID:AB_2651128 IRDye 800CW goat anti-rabbit immunoglobulins LI-COR Biosciences Cat#926–32211; RRID:AB_2651127 Bacterial and virus strains Escherichia coli DH5a Lab stock N/A Escherichia coli BL21-CodonPlus (DE3)-RIL Agilent Cat#230245 Multibac baculovirus/insect cell system Zhang Z. et al.40 and Fujimitsu K. et al.15 N/A Chemicals, peptides, and recombinant proteins ATP Sigma-Aldrich Cat#10519979001 ATPgS Calbiochem Cat#119120 [g-P32]ATP, 6000 Ci/mmol, 10 mCi/mL Hartmann Analytic SRP-501 [S35]Met-label, 1000 Ci/mmol, 10 mCi/mL Hartmann Analytic SCIS-103 Cycloheximide Sigma-Aldrich Cat#C7698 Okadaic acid Sigma-Aldrich Cat#O4511 MG132 proteasome inhibitor Calbiochem Cat#474790 Desthiobiotin Sigma-Aldrich Cat#D1411 Lysozyme Sigma-Aldrich Cat#L6876 Benzonase MILLIPORE Cat#70746 Methylated ubiquitin Sigma-Aldrich Cat#662065 3FLAG-Ub-Cyclin B-N70-KA (Ub-K0-Cyclin B) Fujimitsu K. and Yamano H.41 N/A GST-Cyclin BD167 Gift from J. Gannon pGEX Hs cyclin BD167 CDK2-Cyclin A complex Gift from J. Gannon N/A GST-PreScission protease Lab stock N/A 10His-ENSA Lab stock Plasmid stock #1523 (Continued on next page) Cell Reports 43, 114155, May 28, 2024 11
Ubiquitin Proteomics:Article Title: CDK1-PP2A-B55 interplay ensures cell cycle oscillation via Apc1-loop 300 .
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Apc1 (pS314/pS318) Lab stock #25312 via BioGenes Rabbit polyclonal anti-Apc1 (pS358) This paper #27644 via BioGenes Rabbit polyclonal anti-Apc1 (pT378/pS380) This paper #25316 via BioGenes Rabbit polyclonal anti-Apc1 Lab stock #4853 via BioGenes Rabbit polyclonal anti-Apc2 Lab stock #7576 and #7577 via BioGenes Mouse monoclonal anti-Cdc27/Apc3 BD Biosciences 610455; RRID:AB_397828 Mouse monoclonal anti-Apc3 (AF3.1) Gift from J. Gannon N/A Rabbit polyclonal anti-Apc5 Lab stock #3445 via BioGenes Rabbit polyclonal anti-Apc6 Lab stock #3447 via BioGenes Rabbit polyclonal anti-Gwl Lab stock #7387 via BioGenes Rabbit polyclonal anti-XErp1 Lab stock #8344 via BioGenes Mouse monoclonal anti-FLAG (M2) Sigma-Aldrich F1804; RRID:AB_262044 Mouse monoclonal anti-Cyclin B (X121) Gift from J. Gannon N/A Mouse monoclonal anti-Cdc20 (BA8.1) Gift from J. Gannon N/A Mouse monoclonal anti-6xHis Clontech Cat#631212; RRID:AB_2721905 Mouse monoclonal anti-phosphorylated Plx1 (AZ44) Gift from J. Gannon N/A Goat anti-mouse immunoglobulins, HRPconjugated Dako Cat#P0447; RRID:AB_2617137 Swine anti-rabbit immunoglobulins, HRPconjugated Dako Cat#P0217; RRID:AB_2728719 IRDye 680RD goat anti-mouse immunoglobulins LI-COR Biosciences Cat#926–68070; RRID:AB_2651128 IRDye 800CW goat anti-rabbit immunoglobulins LI-COR Biosciences Cat#926–32211; RRID:AB_2651127 Bacterial and virus strains Escherichia coli DH5a Lab stock N/A Escherichia coli BL21-CodonPlus (DE3)-RIL Agilent Cat#230245 Multibac baculovirus/insect cell system Zhang Z. et al.40 and Fujimitsu K. et al.15 N/A Chemicals, peptides, and recombinant proteins ATP Sigma-Aldrich Cat#10519979001 ATPgS Calbiochem Cat#119120 [g-P32]ATP, 6000 Ci/mmol, 10 mCi/mL Hartmann Analytic SRP-501 [S35]Met-label, 1000 Ci/mmol, 10 mCi/mL Hartmann Analytic SCIS-103 Cycloheximide Sigma-Aldrich Cat#C7698 Okadaic acid Sigma-Aldrich Cat#O4511 MG132 proteasome inhibitor Calbiochem Cat#474790 Desthiobiotin Sigma-Aldrich Cat#D1411 Lysozyme Sigma-Aldrich Cat#L6876 Benzonase MILLIPORE Cat#70746 Methylated ubiquitin Sigma-Aldrich Cat#662065 3FLAG-Ub-Cyclin B-N70-KA (Ub-K0-Cyclin B) Fujimitsu K. and Yamano H.41 N/A GST-Cyclin BD167 Gift from J. Gannon pGEX Hs cyclin BD167 CDK2-Cyclin A complex Gift from J. Gannon N/A GST-PreScission protease Lab stock N/A 10His-ENSA Lab stock Plasmid stock #1523 (Continued on next page) Cell Reports 43, 114155, May 28, 2024 11
Plasmid Preparation:Article Title: CDK1-PP2A-B55 interplay ensures cell cycle oscillation via Apc1-loop 300 .
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Apc1 (pS314/pS318) Lab stock #25312 via BioGenes Rabbit polyclonal anti-Apc1 (pS358) This paper #27644 via BioGenes Rabbit polyclonal anti-Apc1 (pT378/pS380) This paper #25316 via BioGenes Rabbit polyclonal anti-Apc1 Lab stock #4853 via BioGenes Rabbit polyclonal anti-Apc2 Lab stock #7576 and #7577 via BioGenes Mouse monoclonal anti-Cdc27/Apc3 BD Biosciences 610455; RRID:AB_397828 Mouse monoclonal anti-Apc3 (AF3.1) Gift from J. Gannon N/A Rabbit polyclonal anti-Apc5 Lab stock #3445 via BioGenes Rabbit polyclonal anti-Apc6 Lab stock #3447 via BioGenes Rabbit polyclonal anti-Gwl Lab stock #7387 via BioGenes Rabbit polyclonal anti-XErp1 Lab stock #8344 via BioGenes Mouse monoclonal anti-FLAG (M2) Sigma-Aldrich F1804; RRID:AB_262044 Mouse monoclonal anti-Cyclin B (X121) Gift from J. Gannon N/A Mouse monoclonal anti-Cdc20 (BA8.1) Gift from J. Gannon N/A Mouse monoclonal anti-6xHis Clontech Cat#631212; RRID:AB_2721905 Mouse monoclonal anti-phosphorylated Plx1 (AZ44) Gift from J. Gannon N/A Goat anti-mouse immunoglobulins, HRPconjugated Dako Cat#P0447; RRID:AB_2617137 Swine anti-rabbit immunoglobulins, HRPconjugated Dako Cat#P0217; RRID:AB_2728719 IRDye 680RD goat anti-mouse immunoglobulins LI-COR Biosciences Cat#926–68070; RRID:AB_2651128 IRDye 800CW goat anti-rabbit immunoglobulins LI-COR Biosciences Cat#926–32211; RRID:AB_2651127 Bacterial and virus strains Escherichia coli DH5a Lab stock N/A Escherichia coli BL21-CodonPlus (DE3)-RIL Agilent Cat#230245 Multibac baculovirus/insect cell system Zhang Z. et al.40 and Fujimitsu K. et al.15 N/A Chemicals, peptides, and recombinant proteins ATP Sigma-Aldrich Cat#10519979001 ATPgS Calbiochem Cat#119120 [g-P32]ATP, 6000 Ci/mmol, 10 mCi/mL Hartmann Analytic SRP-501 [S35]Met-label, 1000 Ci/mmol, 10 mCi/mL Hartmann Analytic SCIS-103 Cycloheximide Sigma-Aldrich Cat#C7698 Okadaic acid Sigma-Aldrich Cat#O4511 MG132 proteasome inhibitor Calbiochem Cat#474790 Desthiobiotin Sigma-Aldrich Cat#D1411 Lysozyme Sigma-Aldrich Cat#L6876 Benzonase MILLIPORE Cat#70746 Methylated ubiquitin Sigma-Aldrich Cat#662065 3FLAG-Ub-Cyclin B-N70-KA (Ub-K0-Cyclin B) Fujimitsu K. and Yamano H.41 N/A GST-Cyclin BD167 Gift from J. Gannon pGEX Hs cyclin BD167 CDK2-Cyclin A complex Gift from J. Gannon N/A GST-PreScission protease Lab stock N/A 10His-ENSA Lab stock Plasmid stock #1523 (Continued on next page) Cell Reports 43, 114155, May 28, 2024 11
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